Ntrk3 polyclonal antibody
产品名称： Ntrk3 polyclonal antibody
英文名称： Ntrk3 polyclonal antibody
产品编号： PAB11334
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of Ntrk3.
- Immunogen:
- A synthetic peptide corresponding to C-terminus of mouse Ntrk3.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse, Rat
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:5000-1:20000)
Western Blot (1:1000-1:10000)
Immunoprecipitation (1 ug)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- A kinase-deficient TrkC receptor isoform activates Arf6-Rac1 signaling through the scaffold protein tamalin.
Esteban PF, Yoon HY, Becker J, Dorsey SG, Caprari P, Palko ME, Coppola V, Saragovi HU, Randazzo PA, Tessarollo L.J Cell Biol. 2006 Apr 24;173(2):291-9.
- 2.
- Evidence for a role of truncated trkC receptor isoforms in mouse development.
Palko ME, Coppola V, Tessarollo L.J Neurosci. 1999 Jan 15;19(2):775-82.
- 3.
- Differential expression of TrkC catalytic and noncatalytic isoforms suggests that they act independently or in association.
Menn B, Timsit S, Calothy G, Lamballe F.J Comp Neurol. 1998 Nov 9;401(1):47-64.
- Applications
- Western Blot (Tissue lysate)
- Western blot using Ntrk3 polyclonal antibody (Cat # PAB11334) to detect endogenous Ntrk3 in mouse cortex lysate (Lane 1). Lane 2 is Ntrk3 knock-out cortex lysate.
Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
- Western Blot (Transfected lysate)
- Western blot using Ntrk3 polyclonal antibody (Cat # PAB11334) to detect over-expressed Ntrk3 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control.
Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
- Immunoprecipitation
- Mouse cortex lysate was immunoprecipitated with Ntrk3 polyclonal antibody (Cat # PAB11334) and further blotted with affinity purified anti-Ntrk3.
Lane 1 is wild-type cortex lysate, Lane 2 is Grasp knock-out cortex lysate, and Lane 3 is Ntrk3 knock-out cortex lysate.
The membrane was probed with the primary antibody at a 1:6,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.